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. Author manuscript; available in PMC: 2012 Feb 5.
Published in final edited form as: Virology. 2010 Dec 4;410(1):216–227. doi: 10.1016/j.virol.2010.11.012

Figure 3. A chimeric virus that encodes the RRV DC5692 3′UTR in the T48 genetic background (RR67) caused disease in mice that was indistinguishable from RRV T48-induced disease.

Figure 3

(A) Alignment of the 3′ UTR of the RRV strains DC5692 and T48. The underlined TAA (first row) is the E1 stop codon. The italicized and underlined sequences are the repeat elements present in the T48 3′ UTR. (B) Schematic representations of the T48 derived (white) and DC5692 derived (grey) sequence within the genomes of RR64 and RR67. (C) Twenty-four-day-old C57BL/6J mice were inoculated with 103 PFU of RR64 (n = 4), RRV strain DC5692 (n = 4), or RR67 (n = 4) by injection in the left rear footpad. Mice were scored for the development of disease signs including loss of gripping ability, hind-limb weakness, and altered gait. Each data point represents the arithmetic mean ± SD. No statistically significant differences were detected. (D) At 10 dpi, mice were sacrificed and perfused with 4% paraformaldehyde. 5 micron-thick paraffin-embedded sections generated from quadriceps muscles were stained with H&E. Images are representative of four mice per group.