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. Author manuscript; available in PMC: 2012 Feb 5.
Published in final edited form as: Virology. 2010 Dec 4;410(1):216–227. doi: 10.1016/j.virol.2010.11.012

Figure 5. Chimeric viruses that encode the RRV DC5692 PE2 coding region in the T48 genetic background are attenuated in vivo.

Figure 5

(A) Twenty-four-day-old C57BL/6J mice were inoculated with 103 PFU of RR64, RR73, RR78, RR101, RR102, or RR100 by injection in the left rear footpad. At 10 dpi, mice were sacrificed and perfused with 4% paraformaldehyde. 5 micron-thick paraffin-embedded sections generated from quadriceps muscles were stained with H&E. Images are representative of three mice per group. (B) Twenty-four-day-old C57BL/6J mice were inoculated with 103 PFU of RR64 (n = 10), RR73 (n = 7), RR78 (n = 4), RR101 (n = 3), RR102 (n = 3), or RR100 (n =7) by injection in the left rear footpad. Mice were scored for the development of disease signs including loss of gripping ability, hind-limb weakness, and altered gait. Each data point represents the arithmetic mean ± SEM. Data are combined from three independent experiments and were evaluated for statistically significant differences by ANOVA (P < 0.0001) followed by Bonferroni’s Multiple Comparison Test (see text).