Table 1.
Proteins identified by 1D-SG-CID in the EGFR TAP from PC9 (n = 4). Proteins groups identified in the GFP negative control are subtracted from the data set. Proteins shown in bold italics were observed in the GFP TAP, but were enhanced in the EGFR TAP (1D-SG-CID, one-dimensional shotgun collision-induced dissociation; PCT, peptide counts; SCT, spectral counts, SCV, sequence coverage).
| ACCESSION CODE |
SWISSPROT IDENTIFIER |
PCT | SCT | SCV | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| TOTAL | AVERAGE | STD. DEV. | TOTAL | AVERAGE | STD. DEV. | TOTAL | AVERAGE | STD. DEV. | ||
| P00533-1 | EGFR | 61 | 56.75 | 3.4 | 3916 | 979 | 110.21 | 0.54 | 0.52 | 0.02 |
| P11021 | GRP78 | 27 | 22 | 2.94 | 382 | 95.5 | 8.06 | 0.44 | 0.37 | 0.03 |
| P11142-1 | HSP7C | 20 | 18.75 | 0.96 | 345 | 86.25 | 14.2 | 0.38 | 0.36 | 0.03 |
| P62993-1 | GRB2 | 13 | 11.5 | 1 | 332 | 83 | 10.42 | 0.64 | 0.5 | 0.09 |
| Q9UJM3 | ERRFI | 15 | 13.5 | 0.58 | 225 | 56.25 | 7.97 | 0.34 | 0.31 | 0.02 |
| P04626 | ERBB2 | 4 | 2.75 | 0.96 | 207 | 51.75 | 6.02 | 0.04 | 0.03 | 0.01 |
| P08107 | HSP71 | 19 | 17 | 1.41 | 206 | 51.5 | 9.47 | 0.39 | 0.35 | 0.03 |
| Q8TF42 | UBS3B | 21 | 16.25 | 2.22 | 198 | 49.5 | 16.62 | 0.41 | 0.29 | 0.06 |
| P34931 | HS71L | 10 | 9.25 | 0.96 | 150 | 37.5 | 7 | 0.21 | 0.19 | 0.02 |
| P68363 | TBA1B | 10 | 9 | 1.41 | 91 | 22.75 | 4.65 | 0.31 | 0.29 | 0.04 |
| Q9BQE3 | TBA1C | 10 | 8.25 | 1.71 | 86 | 21.5 | 4.43 | 0.32 | 0.27 | 0.05 |
| P07900-1 | HS90A | 9 | 8 | 0.82 | 55 | 13.75 | 2.63 | 0.11 | 0.1 | 0.01 |
| P07437 | TBB5 | 10 | 7 | 0.82 | 49 | 12.25 | 1.5 | 0.33 | 0.21 | 0.03 |
| P68371 | TBB2C | 9 | 6.25 | 0.96 | 46 | 11.5 | 1.73 | 0.3 | 0.18 | 0.04 |
| P08238 | HS90B | 7 | 6.75 | 0.5 | 46 | 11.5 | 1.29 | 0.09 | 0.08 | 0 |
| P68366 | TBA4A | 7 | 6 | 1.15 | 43 | 10.75 | 2.63 | 0.23 | 0.2 | 0.04 |
| P12236 | ADT3 | 6 | 4.25 | 0.96 | 36 | 9 | 1.15 | 0.21 | 0.14 | 0.04 |
| P49411 | EFTU | 7 | 3.75 | 2.5 | 17 | 4.25 | 2.99 | 0.18 | 0.09 | 0.06 |
| Q96CW1-1 | AP2M1 | 3 | 3 | 0 | 14 | 3.5 | 0.58 | 0.06 | 0.06 | 0 |
| P62829 | RL23 | 2 | 2 | 0 | 13 | 3.25 | 0.96 | 0.21 | 0.21 | 0 |
| P38646 | GRP75 | 4 | 2 | 1.41 | 10 | 2.5 | 1.73 | 0.08 | 0.04 | 0.03 |
| P27348 | 1433T | 4 | 2.25 | 0.5 | 10 | 2.5 | 0.58 | 0.16 | 0.08 | 0.03 |
| Q16543 | CDC37 | 2 | 1.5 | 1 | 10 | 2.5 | 2.08 | 0.08 | 0.06 | 0.04 |
| P62988 | UBIQ | 2 | 1 | 1.15 | 8 | 2 | 2.31 | 0.29 | 0.14 | 0.17 |
| P29353-1 | SHC1 | 3 | 1.5 | 1.73 | 6 | 1.5 | 1.73 | 0.05 | 0.03 | 0.03 |
| Q00839-1 | HNRPU | 2 | 1 | 1.15 | 5 | 1.25 | 1.5 | 0.03 | 0.01 | 0.02 |
| P31947-1 | 1433S | 2 | 1 | 1.15 | 5 | 1.25 | 1.5 | 0.06 | 0.03 | 0.04 |
| P42677 | RS27 | 2 | 1 | 1.15 | 4 | 1 | 1.15 | 0.29 | 0.14 | 0.16 |
| P20700 | LMNB1 | 2 | 0.5 | 1 | 3 | 0.75 | 1.5 | 0.03 | 0.01 | 0.02 |
| Q00325-1 | MPCP | 2 | 0.5 | 1 | 3 | 0.75 | 1.5 | 0.05 | 0.01 | 0.03 |
| O94973-1 | AP2A2 | 2 | 0.5 | 1 | 3 | 0.75 | 1.5 | 0.02 | 0.01 | 0.01 |
| Q71UM5 | RS27L | 2 | 0.5 | 1 | 2 | 0.5 | 1 | 0.29 | 0.07 | 0.14 |