Table 2.
Proteins identified by 1D-SG-CID in the EGFR TAP from HCC827 (n = 4). Proteins groups identified in the GFP negative control are subtracted from the data set. Proteins shown in bold italics were observed in the GFP TAP, but were enhanced in the EGFR TAP (EGFR, epidermal growth factor receptor; TAP, tandem affinity purification; GFP, green fluorescent protein; 1D-SG-CID, one-dimensional shotgun collision-induced dissociation; PCT, peptide counts; SCT, spectral counts, SCV, sequence coverage).
| ACCESSION CODE |
SWISSPROT IDENTIFIER |
PCT | SCT | SCV | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| TOTAL | AVERAGE | STD. DEV. | TOTAL | AVERAGE | STD. DEV. | TOTAL | AVERAGE | STD. DEV. | ||
| P00533-1 | EGFR | 58 | 56.5 | 0.58 | 3308 | 827 | 36.89 | 0.51 | 0.51 | 0 |
| P11142-1 | HSP7C | 20 | 18 | 0.82 | 312 | 78 | 6.48 | 0.38 | 0.35 | 0.03 |
| P11021 | GRP78 | 17 | 13.75 | 0.5 | 177 | 44.25 | 3.4 | 0.33 | 0.28 | 0.01 |
| P08107 | HSP71 | 10 | 7.75 | 2.06 | 98 | 24.5 | 4.8 | 0.19 | 0.14 | 0.04 |
| P34931 | HS71L | 6 | 5.25 | 0.5 | 90 | 22.5 | 3 | 0.12 | 0.1 | 0.01 |
| P08238 | HS90B | 14 | 12 | 0.82 | 88 | 22 | 2 | 0.2 | 0.17 | 0.01 |
| P07900-1 | HS90A | 12 | 9.5 | 0.58 | 80 | 20 | 2.58 | 0.17 | 0.13 | 0.01 |
| P62993-1 | GRB2 | 8 | 6.5 | 0.58 | 55 | 13.75 | 1.71 | 0.35 | 0.3 | 0.02 |
| Q9UJM3 | ERRFI | 10 | 7.75 | 0.5 | 45 | 11.25 | 1.5 | 0.25 | 0.21 | 0.02 |
| Q16543 | CDC37 | 3 | 2.25 | 0.5 | 15 | 3.75 | 1.5 | 0.1 | 0.09 | 0.01 |
| P40763-1 | STAT3 | 4 | 1.5 | 1.91 | 6 | 1.5 | 1.91 | 0.08 | 0.03 | 0.04 |
| P49411 | EFTU | 3 | 1.25 | 1.5 | 5 | 1.25 | 1.5 | 0.06 | 0.02 | 0.03 |
| P42677 | RS27 | 2 | 0.5 | 1 | 2 | 0.5 | 1 | 0.29 | 0.07 | 0.14 |
| Q00325-1 | MPCP | 2 | 0.5 | 1 | 2 | 0.5 | 1 | 0.05 | 0.01 | 0.03 |