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. Author manuscript; available in PMC: 2012 Jan 7.
Published in final edited form as: Circ Res. 2010 Nov 4;108(1):40–50. doi: 10.1161/CIRCRESAHA.110.230607

Figure 7.

Figure 7

Effects of Csn8 deficiency on cardiomyocyte apoptosis. A, Representative confocal micrographs of TUNEL-stained (green) myocardial sections from 3-week old mice. The sections were counterstained for F-actin (red) with rhodamine phalloidin and for the nucleus (blue) with TO-RPO 3. To challenge the hearts, mice were treated with isoproterenol (ISO, i.p., 15 mg/kg) 24 hours before being sacrificed. Scale bar=20 μm. B, Quantification of TUNEL-positive cardiomyocyte nuclei. N = 3 mice/group. *: p<0.05, #: p<0.01. C, Representative images of western blot analysis of full length (FL) and cleaved caspase (Casp.) 3 and Bcl2 in ventricular myocardium from 3-week-old mice. D, Kaplan-Meier survival analysis of the impact of cardiac overexpression of Bcl-2 on CR-Csn8KO mice.