Figure 5.

MLK3 is required for merlin suppression of cell proliferation and invasiveness. (a) T29 cells were transfected with nonspecific, human merlin, human MLK3, or human merlin plus human MLK3 siRNA. Cell proliferation was determined by counting cells at various time intervals after transfection. Experiments were performed in triplicate and repeated three times. Cell extracts were prepared from cells at each time interval and analyzed by Western blotting with the indicated antibodies. (b) SW10 cells were transfected with nonspecific siRNA oligo, merlin siRNA, or both MLK3 and merlin siRNAand cell invasiveness was assessed using a modified Boyden chamber. Cell extracts were analyzed by Western blotting with the indicated antibodies.