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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Cryobiology. 2010 Oct 20;61(3):280–288. doi: 10.1016/j.cryobiol.2010.09.006

Table 2.

Quantitative measurements of the radius of histological zones following cryosurgery

Treatment Vascular Stasisb Cryolesion (Histology)c Central Necrosisd Inflammationd Thrombosis /ischemic necrosisd Granulationd Viable Tumord
Day 1 Cryo 2.7±0.7 e 0.9±0.6 2.6±0.9 0.1±0.1 NA 1.4±0.3
TNF+Cryo 3.8±0.8* 3.9±0.6 NA 3.4±0.6 0.5±0.5 NA 1.1±0.2
Day 3 Cryo 3.3±0.5# 3.3±0.6 1.0±0.7 1.9±0.8 0.5±0.4 NA 1.7±0.6
TNF+Cryo 4.0±0.4* 4.3±0.3* 0.5±0.9 3.0±0.5* 0.2±0.2 0.6±0.5 0.7±0.3*
Day 7 Cryo 3.1±0.1 3.2±0.4 0.5±0.3 1.1±0.7 0.7±0.5 0.8±0.3 1.8±0.4
TNF+Cryo 3.7±0.5* 3.8±0.5* NA 2.4±0.5* 0.8±0.4 0.6±0.5 1.2±0.5*
a

The values represent Mean ± SD of three to four Independent experiments for each treatment.

b

Radius of vascular stasis was measured by intravital microscopic imaging following FITC-dextran injection.

c

Radii of granulation tissue (outer bound of cryolesion) were measured from digitized H&E-stained sections (corrected for tissue shrinkage after histologic processing as described in Materials and Methods).

d

Radii of histological zones of central necrosis, inflammation, thrombosis/ischemic necrosis, granulation tissue influx, and viable tumor were measured from digitized H&E-stained sections (corrected for tissue shrinkage after histologic processing as described in Materials and Methods).

e

Cryolesion edge at day 1 after cryosurgery is imperceptible based on H&E-stained sections.

#

There was a significant change (p<0.05) in radius of vascular stasis from day 1 to day 3 as measured by intravital microscopic imaging.

*

Significant changes (p<0.05) between combinatorial (TNF-a + cryosurgery) therapy versus cryosurgery alone were seen in the radii of vascular stasis measured by intravital microscopic imaging, and in radii of histologic zones following cryosurgery.