Table 3.

Changes in expression of DSC1, DSG1 and DHRS7C levels between polled, horned and scurs evaluated by q-PCR.

Contrasts of interest	Gene	ΔΔCt*	s.e.	p-value	q-PCR Fold change#	Microarray fold change#
PvH	DSC1	-3.48	1.2	0.0075	11.2	8.76
PvH	DSG1	-2.95	1.4	0.0402	7.7	7.57
PvH	DHRS7C	-7.36	0.6	<0.0001	164	12.6
PvS	DSC1	0.99	1	0.3213	-1.98	-0.927
PvS	DSG1	0.81	0.9	0.359	-1.87	1.67
PvS	DHRS7C	-5.22	1.1	<0.0001	37.3	3.76
HvS	DSC1	4.47	1.2	0.0004	-22.2	-9.5
HvS	DSG1	3.75	1.2	0.0039	-13.5	-4.56
HvS	DHRS7C	2.13	1	0.0459	-4.38	-3.35

*sign of the difference is based on the reference gene corrected 1^st phenotype Ct minus the reference gene corrected 2^nd phenotype Ct in each contrast for respective target genes.

# fold change is relative to the first phenotype e.g. a change of 11.2 in PvH would indicate that the gene showed 11.2 fold higher expression in polled versus horned tissues