Table 1.
Kinetic constants for recombinant Arabidopsis PFT*
| PFT substrate |
Km (μM) | Specific Activity (pmol min-1 mg-1) |
kcat (hr-1) | kcat/Km (μM-1 hr-1) |
n |
|---|---|---|---|---|---|
| GFP-BD-CVIM | 5.4 +/- 0.6 | 5200 +/- 700 | 28.8 +/- 4.1 | 5.3 +/- 1.0 | 8 |
| GFP-BD-CVIQ | 5.5 +/- 0.9 | 8900 +/- 1900 | 49.1 +/- 10.5 | 8.9 +/- 2.4 | 8 |
| GFP-BD-CVII | 6.9 +/- 1.0 | 2300 +/- 700 | 12.8 +/- 3.7 | 1.9 +/- 0.6 | 8 |
| GFP-BD-CVIL | 7.0 +/- 1.5 | 670 +/- 140 | 3.7 +/- 0.8 | 0.5 +/- 0.2 | 8 |
Standard errors are given.
*PFT assays were performed in the presence of [1-3H]farnesyl diphosphate.