Table 2.
Kinetic constants for recombinant Arabidopsis PGGT1*
| PGGT1 substrate |
Km (μM) | Specific Activity (pmol min-1 mg-1) |
kcat (hr-1) | kcat/Km (μM-1 hr-1) |
n |
|---|---|---|---|---|---|
| GFP-BD-CVIL | 17.2 +/- 4.4 | 110 +/- 30 | 0.50 +/- 0.12 | 0.029 +/- 0.010 | 7 |
| GFP-BD-CVII | 19.0 +/-14.4 | 17 +/- 15 | 0.08 +/- 0.07 | 0.004 +/- 0.005 | 4 |
| GFP-BD-CVIQ | 3.5 +/- 0.6 | 1.7 +/- 0.2 | 0.008 +/- 0.001 | 0.002 +/- 0.001 | 8 |
| GFP-BD-CVIM | 5.8 +/- 1.0 | 2.6 +/- 0.3 | 0.012 +/- 0.001 | 0.002 +/- 0.001 | 8 |
Standard errors are given.
*PGGT1 assays were performed in the presence of [1-3H]geranylgeranyl diphosphate.