Figure 2.

In vivo footprinting of the EgCAD2 promoter. (A) DMS footprint profiles were investigated in the -348/-90 promoter region. (B) Enlargement of the -203/-129 region. The protected (P) or hypersensitive (H) residues in exponentially growing Eucalyptus suspension-cultured cells (8-day-old) and stationary phase cells (15-day-old) are revealed by comparison with in vitro-treated genomic DNA (control). (C) Sequence of the regulatory region. Grey boxes correspond to the MYB sites (MYBa and MYBb) and white boxes correspond to BSa and BSb sites (see text). Representative footprints of eight independent experiments are shown.