Figure 4.

Protein binding activities of the regulatory regions of EgCAD2 and EgCCR promoters containing mutated cis-element(s). EMSAs were performed with tobacco BY2 cell protein extracts (10 μg) and radiolabelled EgCAD2 [-203/-129] or EgCCR [-119/-70] fragments containing individual or multiple mutated cis-elements, as indicated above the lanes by crossed boxes. Lanes 2, 3, 13 and 14 each contain a 100-fold molar excess of unlabelled non-specific (ns) or specific (s) competitor fragment. Complexes are labelled H for high and L for low (see text).