Figure 3.

Multiple sequence alignment of three collinear gene island genes (TaEP1 (A), TaCRP1 (B) and TaZFN1(C)) using CLUSTALX [78]. Each of the wheat, Brachypodium and maize genes were annotated in this study, while the S. bicolor (v1.0 annotations from http://www.phytozome.net/sorghum) and rice annotations [38]. Annotated on the TaCRP1 alignment is the signal peptide and cysteine-rich domain structures outlined in [40]. Colors found on the figure are determined by the CLUSTAL color scheme, which is based on amino acid types.