Figure 1.

Comparison of chitinase activity banding patterns of proteins extracts from control plants (C) and Trichoderma-inoculated (T) plants. As described in the materials and methods, polyacrylamide gels with equal loading in each lane were removed from the glass plates following electrophoresis, subjected to various washes, and stained for chitinase activities using methylumbelliferyl substrates that were dissolved in 100 mM acetate buffer (pH 5.0) containing 1% low melting agarose. Chitinase activity bands from shoots (A) and roots (B) are shown. Activity bands started to appear as early as 2 min after beginning of incubation. Different intensities were observed for the activity bands in control and Trichoderma-inoculated plants, as shown in (C) -shoots and (D) -roots. The bands observed were numbered: 1-5 in shoots and 6-8 in roots; Bands intensities were measured and compared between the two treatments. Molecular size markers: 106, 93, 52, 32, 28, and 18 kDa.