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. 2010 May 7;10:84. doi: 10.1186/1471-2229-10-84

Table 2.

Sequences of the PCR primers used in this work.

CLONING OF CODING SEQUENCES
TCRR2-GWS AAAAAGCAGGCTCCATGGACACTATTGGTCCAC

TCRR2-GWAS AGAAAGCTGGGTATCAAATATAGCTCAAGATACGAGG

HP1.3-GWS AAAAAGCAGGCTCCATGTCTGCCGCGAACCAGC

HP1-GWAS AGAAAGCTGGGTTACTTGTTGGGGGGAAAGC

HP3-GWAS AGAAAGCTGGGTCACTTGCTGGGGGGAC

HP2-GWS AAAAAGCAGGCTCCATGGCTGCCGCCGCGC

HP2-GWAS AGAAAGCTGGGTTATTGTTGAGCCTGGATTTGC

AttB recombination motifs are shown underlined

CLONING OF ZmTCRR-1 AND ZmTCRR-2 PROMOTER

TCRR1p-GWS AAAAAGCAGGCTAGCTTCATAGGATGATCCAC

TCRR1p-GWAS AGAAAGCTGGGTGGACTAGCTAGACAAGCTC

TCRR2p-GWS AAAAAGCAGGCTTAGTGTGCAATCGAAGCAACGG

TCRR2p-GWAS AGAAAGCTGGGTAGATACTCTCCCACAACTTCC

AttB recombination motifs are shown underlined

qRT-PCR PRIMERS

HP1.3-QPCRS AACACTTGCATTCAGTTCCGCG

HP1-QPCRAS CCAGTACCTTGAGGCACCCATCTC

HP3-QPCRAS CCAGTGTCTTGAGGCACCCATCTT

HP2-QPCRS TCGTCACCCTCTTCTGCGACG

HP2-QPCRAS CCACGATGGGCTGGTCAAGC

TCRR2-QPCRS ATTCAAGTGACAATGGTGGAGGG

TCRR2-QPCRAS CCAGGCATACAATAATCGGTCAGA

FKBP-QPCRS GGGTGCTGTTGTTGAAGTCA

FKBP-QPCRAS GCAATAACTTCCTCTTCATCG

HK1-QPCRS GTGTGGCAGAGCATTGATTACAC

HK1-QPCRAS TCACATACAAATACGGCAAGCTCA

HK1a2-QPCRS GTGTGGCAGAGCATTGATAACGT

HK1a2-QPCRAS ACTGCAAGCTCAATGCACTTCTCC

HK1b1-QPCRS AATGGCAGTTCTCTAACCAGCACG

HK1b1-QPCRAS TTTTGGGCAATCCAGGTGGACC

HK1b2-QPCRS CGCTAATCAATGAAGTGCTTGACAG

HK1b2-QPCRAS GATTCAAGATCCAACTTTCTGGC

HK2-QPCRS CACAGGAGAAAGGACTGGAGTTGG

HK2-QPCRAS GGATCGCCAATTAGTGTTTGTGG

HK3A-QPCRS GTCATGCACCTGCAGTATTGG

HK3A-QPCRAS TACATAAGTCACATTCATGCGGATT

HK3B-QPCRS GCGATCGGCAGCATATTTGGAA

HK3B-QPCRAS GCTGCGGAAACCAGACCAAAC

C4pepQPCR-S GAGGCTCTGCAGAGAGAGATCC

C4pepQPCR-AS CCATAGCGCATTTCGGCCTG

ZmRR2QPCR-S GCGCAGCTCCAAGTACAGAGTGAC

ZmRR2QPCR-AS TGTTCACGTCGGGGACCAGC