Table 3.
Microsatellite markers (described by Billotte et al. [27]) used for a larger-scale survey for hemizygosity of Hs and homozygosity of DH candidates previously identified by the morphological screen, microsatellite pre-screen (15 markers) and flow cytometry screen.
| No. | Forward primer (5'-3') | Reverse primer (5'-3') |
|---|---|---|
| 16 | GACCTTTGTCAGCATACTTGGTGTG | GCAGGCCTGAAATCCCAAAT |
| 17 | ATGCATGTGATTTTATTAGGTGAGA | CGACCCTCAGTCAATCAGTAAG |
| 18 | AAGCTAGCGACCTATGATTTTAGA | AAACAAGTAATGTGCATAACCTTTC |
| 19 | CCCACCACCCCTAGCTTCTC | ACCCCGGTCCAAATAAAATC |
| 20 | AGAGAGAGAGAGTGCGTATG | GTCCCTGTGGCTGCTGTTTC |
| 21 | GGGTAGCAAACCTTGTATTA | ACTTCCATTGTCTCATTATTCT |
| 22 | CGAGGCCCAAAAACATTCAC | GGTCCCGATCCCGTCTACTG |
| 23 | TTGCGGCCCATCGTAATC | TCCCTGCAGTGTCCCTCTTT |
| 24 | AGGGAATTGGAAGAAAAGAAAG | TCCTGAGCTGGGGTGGTC |
| 25 | AGCAAGAGCAAGAGCAGAACT | CTTGGGGGCTTCGCTATC |
| 26 | TAGCCATGCCGCCACCACTT | CAATCCATTAGCGTGCCCTTCT |
| 27 | CTTACCCCGCCTCCTCTCCT | CGAAATGCCCTTCCTTTACACTA |
| 28 | CCTTATATCGCACGGGTTCC | TTCTTGGGGTCTCGCTACGG |
| 29 | GCAAGATGCAATGGAGTTCA | CAAACCGCAGCAAGTCAGA |
| 30 | GCAAAATTCAAAGAAAACTTA | CTGACAGTGCAGAAAATGTTATAGT |
| 31 | CGTTCATCCCACCACCTTTC | GCTGCGAGGCCACTGATAC |
| 32 | GAATGTGGCTGTAAATGCTGAGTG | AAGCCGCATGGACAACTCTAGTAA |
| 33 | ACATTCCCTCTATTATTCTCAC | GTTTTGTTTGGTATGCTTGT |
| 34 | AAGCCAACTTCACAGATATGTTGAT | ATGAGCCTAACAAAGCACATTCTAA |
| 35 | AGTGAGGTATGGTTGATTAGGA | TATTGATAGCATTTGGGATTAG |
| 36 | CTCCGATGGTCAAGTCAGA | AAATGGGGAAGGCAATAGTG |
| 37 | GCCGTTCAAGTCAATTAGAC | TTTGGGAGCAAGCATTATCA |
| 38 | TGCTTCTTGTCCTTGATACA | CCACGTCTACGAAATGATAA |
| 39 | CACCACATGAAGCAAGCAGT | CCTACCACAACCCCAGTCTC |
| 40 | TTTTATTTTCCCTCTCTTTTGA | ATTGCGTCTCTTTCCATTGA |
| 41 | CATATGGCGCACAGGCAC | GCAATACAAGAGCACCCAAAT |
| 42 | AGTTGGTTTGCTGATTTG | TGTTGCTTCTTTGATTTTC |
| 43 | GCTGAAGATGAAATTGATGTA | TTCAGGTCCACTTTCATTTA |
| 44 | ATGACCTAAAAATAAAATCTCAT | ACAGATCATGCTTGCTCACA |
| 45 | GGTGCAAGAGAGGAGGAATG | TTTGGTAGTCGGGCGTTTTA |
| 46 | GTTTGGCTTTGGACATG | TCCATCACAGGAGGTATAG |
| 47 | TGTTTTGTTTCGTGCATGTG | GGCTGACATGCAACACTAAC |
| 48 | CGGTTTTGTCGCATCTATG | GTCGTCAGGGAACAACAGT |
| 49 | CAATCATTGGCGAGAGA | CGTCACCTTTCAGGATATG |
| 50 | GAGCATGACGCAAACAAAGG | GCAACATGTTTGATGCATTAATAGTC |
| 51 | TCCAAGTAGCAAATGATGAC | TGCCCTGAAACCCTTGA |
| 52 | GAAGGGGCATTGGATTT | TACCTATTACAGCGAGAGTG |
| 53 | AACACTCCAGAAGCCAGGTC | GGTTTAGGTATTGGAACTGATAGAC |
| 54 | GATCCCAATGGTAAAGACT | AAGCCTCAAAAGAAGACC |
| 55 | TGTGGTTTGAGGCATCTTCT | GCCCACCAAAAGAAAGTAGT |
| 56 | TAGCCGCACTCCCACGAAGC | CCAGAATCATCAGACTCGGACAG |
| 57 | TCAAAGAGCCGCACAACAAG | ACTTTGCTGCTTGGTGACTTA |
| 58 | GGGGATGAGTTTGTTTGTTC | CCTGCTTGGCGAGATGA |
| 59 | TCTAATGCTCCCAAGGTACA | GGCTTGGTCCACGATCTT |
| 60 | AGCTCTCATGCAAGTAAC | TTCAACATACCGTCTGTA |
| 61 | TCCTCACTGCTCCTCTAATC | ACTCCCTATGGACCTTAGTC |
| 62 | AGGGAGGCGAACGAGAAACA | CGACTGCTGATGGGGAAGAG |
| 63 | CTACGGACTCACACCTATAT | ATGGTTCATCAATGAGATC |
| 64 | GTGAGCGATTGAGGGGTGTG | GGGGCTTGATTGAGTATTTCCA |
| 65 | AGGGCAAGTCATGTTTC | TATAAGGGCGAGGTATT |
| 66 | GAAGCCTGAGACCGCATAGA | TTCGGTGATGAAGATTGAAG |
| 67 | TTTCTTATGGCAATCACACG | GGAGGGCAGGAACAAAAAGT |
| 68 | GTTTATCATTTTGGGGTCAG | CGGTGTCCCTCAGGATGTA |
| 69 | CATGCACGTAAAGAAAGTGT | CCAAATGCACCCTAAGA |
| 70 | AATCCAAGTGGCCTACAG | CATGGCTTTGCTCAGTCA |
| 71 | TGTAGGTGGTGGTTAGG | TGTCAGACCCACCATTA |
| 72 | AGCAAGACACCATGTAGTC | GACACGTGGGATCTAGAC |
| 73 | AAAAGCCGATAGTGGGAACA | ATGCTGAGAGGTGGAAAATAGAG |
| 74 | GTCCATGTGCATAAGAGAG | CTCTTGGCATTTCAGATAC |
| 75 | AGCCAATGAAGGATAAAGG | CAAGCTAAAACCCCTAATC |
| 76 | CAATTCCAGCGTCACTATAG | AGTGGCAGTGGAAAAACAGT |
| 77 | GGGCTTTCATTTTCCACTAT | GCTCAACCTCATCCACAC |
| 78 | GACAGCTCGTGATGTAGA | GTTCTTGGCCGCTATAT |
| 79 | ACTTGTAAACCCTCTTCTCA | GTTTCATTACTTGGCTTCTG |
| 80 | CCTTCAAGCAAAGATACC | GGCACCAAACACAGTAA |
| 81 | CCACTGCTTCAAATTTACTAG | GCGTCCAAAACATAAATCAC |
| 82 | GGGAGAGGAAAAAATAGAG | CCTCCCTGAGACTGAGAAG |
| 83 | AGCAGGGCAAGAGCAATACT | TTCAGCAGCAGGAAACATC |
| 84 | GCCTATCCCCTGAACTATCT | TGCACATACCAGCAACAGAG |
| 85 | CATCAGAGCCTTCAAACTAC | AGCCTGAATTGCCTCTC |
| 86 | ATTCATTGCCATTCCCTTCA | TTGTCCCCTCTGTTCACTCA |
| 87 | ATTGCAGAGATGATGAGAAG | GAGATGCTGACAATGGTAGA |
| 88 | TCTCCCAAATCACTAGAC | ATCTGCAAGGCATATTC |
| 89 | ACGTTTTGGCAACTCTC | ACTCCCCTCTTTGACAT |
| 90 | TCCACTCTGGCAACTCC | AAGGATGGGCTTTGTAGT |
| 91 | TTTAGAGGACAAGGAGATAAG | CGACCGTGTCAAGAGTG |
| 92 | AGCAAAATGGCAAAGGAGAG | GGTGTGTGCTATGGAAGATCATAGT |
| 93 | GTAGCTTGAACCTGAAA | AGAACCACCGGAGTTAC |
| 94 | AAGCCACCAGGATCATC | GTCATTGCCACCTCTAACT |
| 95 | TTACTTGCTAAGCTCTCTAGC | TGGCTGTTTAATCTGTCTG |
| 96 | TCTATATTTGGTTGGCTTGA | ACTCATTTCAATCTCAGTGTC |
| 97 | TGCTACGTGCTGAAATA | ATTTCAGGTTCGCTTCA |
| 98 | CCTCCACTTCTCTTCATCTT | CTTCCTCAAGCTCAAACAAT |
| 99 | GATGTTGCCGCTGTTTG | CATCCCATTTCCCTCTT |
| 100 | ATGCTCCACCAAGTTTA | CACATCCTAGCATCATTG |
| 101 | AAGCAATATAGGTTCAGTTC | TCATTTTCTAATTCCAAACAAG |
| 102 | GCTCGTTTTTGTTTAGGTGA | TTTTCTCCATAGTCCGTTAC |
| 103 | CAGCACACAAATGACAT | CACCTTTCCTTTTTGTC |
| 104 | CCTATTCCTTACCTTTCTGT | GACTTACTATCTTGGCTCAC |
| 105 | CCTTGCATTCCACTATT | AGTTCTCAAGCCTCACA |
| 106 | CCTCCTTTGGAATTATG | GTGTTTGATGGGACATACA |
| 107 | ATTGGAGAGCACTTGGATAG | TTCTCTTCCTTCTCACTTGT |
| 108 | AGCCAGATGGAAATACAC | GTGCGATAAAGAGGAGAGT |
| 109 | TAGTTTTCCCATCACAGAGT | ACAATATTTAGACCTTCCATGAG |
| 110 | GTGCAGATGCAGATTATATG | CCTTTAGAATTGCCGTATC |
| 111 | ACAATAACCTGAGACAACAAGAAAC | ATACATCCCCTCCCCTCTCT |
| 112 | GAACTTGGCGTGTAACT | TGGTAGGTCTATTTGAGAGT |