Figure 1.

Western blot analysis and submitochondrial location studies of ORFH79. A Western blot analysis of the anther total protein extraction (AN/A,AN/B), and the purified mitochondria protein from etiolated shoots (ES/A,ES/B), and spikelets (SP/A,SP/B). Signals were obtained in the protein fraction from anthers of YtA, purified mitochondria from etiolate seedlings and spikelet of YtA. No signal appeared in any YtB tissues. ES: etiolated shoots, SP: spikelet, AN: anthers. A: YtA, B; YtB. The blot shown is representative of several independent experiments. B Analysis of the submitochondrial location of ORFH79. Protein samples were prepared from the purified mitochondria of YtA. Signal was observed in the insoluble protein fractions. Insol: insoluble fractions of mitochondria, Sol: soluble fractions of mitochondria.