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Segregation of the T1 transgenic plant with orfH79
| Plant lines | Number of positive plant | Number of negative plants | χ2value (for 1:1 segregation) | P |
|---|---|---|---|---|
| U6-2 | 15 | 13 | 0.14 | 0.70-0.90 |
| U6-3 | 10 | 10 | 0.05 | 0.90-0.95 |
| U3-2 | 17 | 13 | 0.3 | 0.50-0.70 |
The presence of transgene was determined using PCR. Positive plants: T1 progeny with orfH79 insert, showing a semi-male-sterile phenotype (50% abortion pollens); Negative plants: T1 progeny without orfH79 insert, and showing a fertile phenotype.
