Table 1.
Distribution of microsatellites in the unigene sequences of sugarcane
| Characters under study | Unigenes* |
|---|---|
| Number of sequences examined | 15,594 |
| Size (bp) of examined sequences | 9,17,43,95 |
| Number of identified perfect microsatellites | 2,712 (17.4) |
| Number of perfect microsatellite containing sequences | 2,230 (14.3) |
| Number of perfect microsatellite (excluding mononucleotides) containing sequences | 584 (3.7) |
| Number of sequences containing more than one perfect microsatellites | 167 (28.6) |
| Number of sequences containing single and unique perfect microsatellites | 417 (71.4) |
| Number of mononucleotides | 1,871 (12) |
| Number of dinucleotides | 200 (23.8) |
| Number of trinucleotides | 615 (73.1) |
| Number of tetranucleotides | 15 (1.8) |
| Number of pentanucleotides | 7 (0.83) |
| Number of hexanucleotides | 4 (0.47) |
| Number of perfect microsatellites excluding mononucleotides | 841 (5.4) |
| Size (kb) of sequences containing one perfect microsatellite | 10.9 |
| Number of perfect class I microsatellites | 207 (24.6) |
| Size (kb) of sequences containing one perfect class I microsatellite | 44.3 |
| Number of primer pairs designed for perfecta microsatellites | 810 (96.3) |
| Number of compound class I microsatellite containing sequences | 183 |
| Size (kb) of sequences containing one compound class I microsatellite | 50.1 |
| Number of compound class I microsatellites | 183 (1.2) |
| Number of compound interrupting class I microsatellites | 137 (74.9) |
| Number of compound non-interrupting class I microsatellites | 46 (25.1) |
| Number of primer pairs designed for compound class I microsatellites | 151 (82.5) |
*The number in the bracket is the proportion expressed in percentage
aMononucleotides to hexanucleotides repeated up to 100 times without any interruption at a locus