Table 1.
Constructs investigated in this study
| Construct name | Protein sequencea | Net chargeb | Localizationc |
|---|---|---|---|
| FcpD-eGFP | ...FAPAKNAARTSVATT-MVSK... | +3 | S |
| FcpD-eGFP_K4E | ...FAPAKNAARTSVATT-MVSE... | +1 | S |
| FcpD-eGFP_K4A | ...FAPAKNAARTSVATT-MVSA... | +2 | S |
| FcpD_K20E+R24E-eGFP_K4E | ...FAPAENAAETSVATT-MVSE... | -3 | ER |
| FcpD_K20R-eGFP_K4R | ...FAPARNAARTSVATT-MVSR... | +3 | S |
| FcpD_K20A+R24A-eGFP_K4A | ...FAPAANAAATSVATT-MVSA... | 0 | PPC |
| FcpD_K20E+R24E-eGFP | ...FAPAENAAETSVATT-MVSK... | -1 | ER/PPC |
| FcpD_K20E+N21K+R24E-eGFP | ...FAPAEKAAETSVATT-MVSK... | 0 | PPC |
| FcpD_K20E+N21K+R24E-eGFP_K4E | ...FAPAEKAAETSVATT-MVSE... | -2 | ER |
| FcpD_K20A+R24A-eGFP | ...FAPAANAAATSVATT-MVSK... | +1 | PPC |
| FcpD_K20E+N21R+R24E-eGFP_K4R | ...FAPAERAAETSVATT-MVSR... | 0 | PPC |
| FcpD_K20E+N21R+R24E-eGFP_K4E | ...FAPAERAAETSVATT-MVSE... | -2 | ER |
| FcpD_Δ20-30-eGFP | ...FAPA-MVSK... | +1 | S |
| FcpD_Δ20-30-eGFP_K4R | ...FAPA-MVSR... | +1 | S |
| FcpD_Δ20-30-eGFP_K4E | ...FAPA-MVSE... | -1 | ER |
| FcpD_Δ20-30-eGFP_K4A | ...FAPA-MVSA... | 0 | PPC |
| FcpD_3xSpacer-eGFP | ...FAPAKNAARTSVATT-[M(GGGGP)5]3-MVSK... | +2 | S |
| FcpD_Δ20-30-Spacer-eGFP | ...FAPA-[M(GGGGP)5]-MVSK... | 0 | S |
| FcpD_Δ20-30-2xSpacer-eGFP | ...FAPA-[m(GGGGP)5]2-MVSK... | 0 | S |
| FcpD_Δ20-30-3xSpacer-eGFP | ...FAPA-[M(GGGGP)5]3-MVSK... | 0 | ER |
| FcpD_Δ20-30-3xSpacer_G4K-saGFP11 | ...FAPA-[M(GGKGP)-(GGGGP)4]3-MVSK... | +1 | ER/S |
aSequence of the FcpD transit peptide, of the eGFP N-terminus and, in spacer constructs, of the synthetic spacers separated by hyphens, respectively; charged amino acids (or substituted alanines) in bold; bCalculated from the bold-faced amino acids, in spacer constructs up to the end of the first spacer; cS: stroma; ER: endoplasmic reticulum; PPC: periplastid compartment.