Table 3.
Primers used in chimeric expression vector construction
| Genes | F/R | Primers Sequence (5'-3') |
|---|---|---|
| orf220 | F | ATGCCTCAACTGGATAAATTCACTT |
| R | TCATCGAAATAGATCGAGGATCTCG | |
| Chimeric gene orf220 | F | TACGGATCCATGCCTCAACTGGAT (BamHI) |
| R | AGAGAGCTCTCATCGAAATAGATC (SacI) | |
| Chimeric gene atp2-1 | F | CCTCTAGACCATGGCTTCTCGGAGGCTTCT (XbaI) |
| R | CCGGATCCGCTGCGGAGGTAGCGTACTG (BamHI) | |
| atp2-1/orf220 GFP | F | CACCATGGCTTCTCGGAGGCTTCT |
| R | TCGAAATAGATCGAGGATCTCG | |
| orf220 GFP | F | CACCATGCCTCAACTGGATAAATTCACTT |
| R | TCGAAATAGATCGAGGATCTCG |
Note: underlined italicized nucleotides represent restriction enzyme sites. F, forward; R, reverse.