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. 2010 Nov 5;10:239. doi: 10.1186/1471-2229-10-239

Table 1.

Media composition for the wheat and barley tissue and suspension cultures.

Component Medium B5 Medium HV
mg/l mg/l

NaH2PO4 × H2O 172

KH2PO4 34

CaCl2 × 2 H2O 150 88

(NH4)2SO4 134

NH4NO3 1650

MgSO4 × 7 H2O 250 370

KNO3 2500 1900

FeSO4 × 7 H2O 25.6 11.12

Na2EDTA × 2 H2O 34.27 15.72

KJ 0.75 0.166

MnSO4 × H2O 10 4.46

H3BO3 3 1.24

ZnSO4 × 7 H2O 3 1.72

Na2MoO4 × 2 H2O 0.25 0.05

CuSO4 × 5 H2O 0.25 0.005

CoCl2 × 6 H2O 0.25 0.005

Nicotinic acid 1 1

Thiamine hydrochloride 10 10

Pyridoxal hydrochloride 1 1

myo-Inositol 100 100

2,4-Dichlorophenoxyacetic acid 2 2

Sucrose 20000 30000

(Agar) (8000) (8000)

pH = 5.5 pH = 6.0

(Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany).

Sterilized at 120°C for 30 minutes