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. 2010 Nov 5;10:239. doi: 10.1186/1471-2229-10-239

Table 2.

Origin of the genes and the primers for the RT-PCR experiments.

Name Primers
Tubulin F2 5' CCTCATCACCGTCCTCGCC 3'
R2 5' TGATCTCAGCTGAGAAGGC 3'

P5CS F1 5' CGTGAAGCGCATCATAATCA 3'
R1 5' ATCAGCCCACTCTGACCAAC 3'

P5CR F1 5' AACGAACCCTTCTCGAGCTCATG 3'
R1 5' AGCAGCATCAGTGATGTGTCTAG 3'

For the primer design NCBI gene bank entries were used (tubulin: T. aestivum tubulin 3A DQ435663, H. vulgare α-tubulin 3 AJ132399; P5CS T. aestivum AB 193551 and H. vulgare AK249154; P5CR: T. aestivum AAW82908 and H. vulgare AY177684).