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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: J Mol Cell Cardiol. 2010 Jun 8;49(4):625–638. doi: 10.1016/j.yjmcc.2010.05.014

Fig. 9.

Fig. 9

Silencing Akt attenuates BFT-induced protection on cardiomyocytes exposed to hypoxia under normal or high glucose. Bar graphs show levels of activated caspase 3/7 (A), pAKt (B), Pim-1(C) and Bcl-2 (D) in cultured adult cardiomyocytes after silencing Akt. Cardiomyocytes cultured in normal (NG) or high glucose (HG) were transfected with Ad. DN-Akt (NGdnAkt and HGdnAkt) and subjected to hypoxia with 0.2% O2 for 18 h after treating the cells with BFT (NGBdnAkt and HGBdnAkt) or vehicle (NGVdnAkt or HGVdnAkt). Values are mean±standard deviation and expressed as relative units (RLU) for caspase 3/7 activity and n-fold changes toward vehicle-treated NG (NGV) for pAkt, Pim-1 and Bcl-2.§P<0.01 and §§P<0.001versus NG or HG; *P<0.01 and **P<0.001 versus vehicle-treated NG (NGV) or HG (HGV); #P<0.01 and ##P<0.001 versus BFT-treated NG (NGB) or HG (HGB); δP<0.01 and δδP<0.001 versus corresponding treatment between NG or HG cultured cells. Each experiment was repeated four times in triplicate except caspase 3/7 activity, which was performed in 6 wells per each condition and repeated 3 times.