Fig. 2.

The SPLASSH Graphical User Interface provides a single window from which to control all aspects of multispectral and fluorescence imaging acquisition. The GUI is organized into panels which contain buttons that access similar types of high-level routines (see Section 3.2 for descriptions). Acquired images are displayed in their own pop-up windows, separate from the control GUI. When initializing the program, SPLASSH dynamically communicates with the camera to determine its capabilities and available camera properties. SPLASSH then updates the corresponding GUI interfaces to reflect these values (e.g. the number of available binning modes).