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. 2010 Aug 2;1(2):453–462. doi: 10.1364/BOE.1.000453

Fig. 5.

Fig. 5

Images of Hela cells (top row), MG63 cells (middle row), and folate receptor blocked Hela cells (bottom row) incubated with SNP-DBF-NHFA (20 µM, 2 h). Left column: DIC, 40 ms. Middle column: one-photon fluorescence image, 200 ms (filter cube Ex: 377/50 DM: 409 Em: 525/40). Right column: 3D reconstruction from overlaid two-photon fluorescence images (Ex: 740 nm; Power: 30 mW; Em. short-pass filter 690 nm) 10 µm grid.