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Images of Hela cells incubated with SNP-DBF-NHFA (20 µM, 2 h). (a) DIC, 20 ms, (b) 3D reconstruction from overlaid two-photon fluorescence images (Ex: 740 nm; Power: 30 mW; Em. short-pass filter 690 nm) 10 µm grid, (c) 2P-FLIM image (Ex: 740 nm; Power: 30 mW).
