Table 3.

Involvement of IL-4 and IFN-γ in anti-LFA-1/ICAM-1-induced cardiac allograft tolerance

Exp.	Recipient	Cell transfer	Survival, days	Mean survival, days
1	WT	–	>100 × 7	>100
	IL-4 KO	–	13, 26, 30, >60, >60, >60	>45
	IFN-γ KO	–	10, 11, 13, 16, 17	13
2	NKTKO	WT	>60, >70, >70	>70
	NKTKO	IL-4 KO	20, 26, 32, >60, >70, >70	>46
	NKTKO	IFN-γ KO	16, 18, 34, 42, >60	34

In experiment (Exp.) 1, WT, IL-4 KO, or IFN-γ KO B6 mice were transplanted with BALB/c heart and then treated with anti-LFA-1/ICAM-1 mAbs. In Exp. 2, splenocytes (1.5 × 10⁷ cells) and bone marrow cells (3 × 10⁷ cells) from WT, IL-4 KO, or IFN-γ KO B6 mice were transferred into NKT KO B6 mice before transplantation of BALB/c heart and anti-LFA-1/ICAM-1 mAbs treatment. Graft survival was monitored daily by palpation.