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. 2010 Aug 2;1(2):414–424. doi: 10.1364/BOE.1.000414

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©2010 Optical Society of America

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.

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Fig. 5 — Comparison of quantitative phase maps with fluorescent assays during an optoinjection experiment: a) phase map and b) propidium iodide fluorescence (optoinjection assay) 5 min after irradiation ; c) phase map and d) Calcein AM fluorescence (viability assay) after 90 min incubation. Two cells were successfully optoinjected - one proved viable (solid arrow) while the other (dashed arrow) was necrotic after 90 min. Note the significant decrease in the optical thickness of the non-viable cell. Scale bars 20 μm.