Fig. 4.

Real time analysis of tear film dynamics in response to instillation of Refresh Tears ® (Allergan, Irvine,CA). The camera acquired images at 300 frames/s. Changes of the thickness of tear film over approximately 7 seconds are displayed here. The experiment starts out with a dried eye. A few seconds into the data acquisition, a drop of Refresh Tear Plus® is instilled into the rabbit eye. The thickness of the tear film increases approximately 10 fold. After another two seconds (when the bulk of the drop is out of the imaging plane), the tear film starts finding a stability in thickness. At this point, the thickness stays at approximately 18µm, and holds this thickness throughout the data acquisition.