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. 2010 Nov 15;11:632. doi: 10.1186/1471-2164-11-632

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Copyright ©2010 Tremblay et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Characterization of the Chromosome 4 and 19 SST1 macrosatellite arrays. (A) Ideogram of chromosome 4 and schematic map of the genomic interval around the array, including a single inverted monomer 210 kb distal. Below is a schematic of a 2.4 kb monomer. The CpG island is marked by the grey box (CGI). KpnI and ApaL1 restriction endonuclease sites are indicated. (B) The y-axis is a 2.4 kb monomer and the x-axis is the 48 kb genomic interval (132.8 Mb hg18). Dot-plot generated using NCBI Blast, and output image labelled in Adobe Photoshop CS2. (C) Ideogram of chromosome 19 and schematic map of 1.6 Mb genomic interval encompassing the arrays. Partial monomers represented by single arrow heads. Black arrows represent genes and direction/extent of transcription. Below is a schematic of an individual 2.5 kb monomer. KpnI and PvuII restriction endonuclease sites are indicated. (D) The y-axis is a single 2.5 kb monomer and the x-axis is the 45 kb (41.4 Mb hg18) or 41 kb (42.4 Mb hg18) genomic intervals. (E) Copy number variation of the chromosome 4/19 macrosatellite arrays. Southern of XbaI cut DNA from 22 unrelated individuals separated by PFGE hybridized with a chromosome 4/19 macrosatellite probe. The ethnicity of the individuals used is indicated at the top. Size markers are given along the left edges in kb. (F) Southern of 11 unrelated individuals genomic DNA cut with either PvuII or ApaLI hybridized with a chromosome 4/19 macrosatellite probe.