Table 1.
Target | Application | Forward | Reverse | Size |
---|---|---|---|---|
Chr5 | Probe | ACTCGTGACGAACCGACTTG | GCTTGTAGGCTTATGTCTGG | 571 bp |
Chr5 | Probe | TGATTCCTCCAGCTGGAAAG | CAGCAAGAAGGATGGAATCC | 789 bp |
Chr13 | Probe | GGAAATCACGGAGACCGCAG | GTTCCATTCCAATCGCCTTG | 472 bp |
Chr4/19 | Probe | GGAGGGTGGGTTTCTTGCAG | AGACGTTGAGCCAGACTAAG | 576 bp |
Chr9 | Probe | GGGCAAATTTCGAGTGAAGG | CCAGTTCCAGCGGTGATTTC | 779 bp |
Chr5 | RT-PCR | CAGGGTTGTCATCCTCTGAG | CAGCAAGAAGGATGGAATCC | 226 bp |
Chr13 | RT-PCR | GGAAATCACGGAGACCGCAG | ACAACGTGGGTGGCAGATAG | 306 bp |
Chr4/19 | RT-PCR | GGGTGGGTTTCTTGCACGTC | CGGGGAAGTCTTCTTTGTGG | 594 bp |
Chr9 | RT-PCR | TGGTTGACGACTCGCGAGAG | CAAAGCACCGGCCTAGATCG | 154 bp |
GAPDH | RT-PCR | GAAGGTGAAGGTCGGAGTC | AGGTCCACCACTGACACGTT | 729 bp |
Primers used in the current study to amplify the macrosatellites listed for probe generation or assessment of expression by RT-PCR analysis.