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. 2001 Feb 20;98(5):2587–2592. doi: 10.1073/pnas.051632398

Figure 4.

Figure 4

Secretion of TGF-β by AND T cells. Purified T cells from AND TCR+ mice were activated as in Fig. 1. At 72 h, supernatants were collected and assayed for the presence of TGF-β by ELISA (A) or bioassay (B). (A) TGF-β production by AND TCR transgenic T cells stimulated with TCR alone (open bars), TCR/CTLA-4 (solid bars), TCR/CD28 (shaded bars), and TCR/CD28/CTLA4 (hatched bars). (B) For the bioassay, a 1:1 dilution of supernatant was added to the cell line Mv1Lu, and the proliferation of these cells was determined at 20 h. T cell activation supernatants were heated at 80°C for 2 min to activate latent TGF-β. Shown is inhibition of proliferation of Mv1Lu cells 20 h after addition of treated supernatants.