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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Immunogenetics. 2010 Sep 2;62(10):701–707. doi: 10.1007/s00251-010-0473-9

Fig. 2. MHC-II DQ restriction of SIV-specific CD4+ T-cell responses is allele specific.

Fig. 2

Three CD4+ T-cell clones specific for the Gag-WP10, Gag-KV10 and Gag-PI16 epitopes derived from animal r02089 were restricted by the DQB1*1801 allele and only the DQA1*2601 or DQA1*2402 alleles (A). DQA1*2601 paired with other DQ beta molecules and specific peptide, did not elicit IFNγ secretion from the same three clones (B). The CD4+ T-cell clone specific for the Gag-LL11 epitope derived from r01099 and restricted by DQB1*1708 also required the DQA1*2402 allele for IFNγ secretion (C). A different DQ beta allele besides DQB1*1708 paired with DQA1*2402 did not elicit IFNγ secretion from the same clone (D). Bars in the four panels show the mean and the standard deviation of results from one representative experiment. Secretion of IFNγ was normalized to each SIV-specific CD4+ T-cell clone when stimulated with homologous BLCLs and relevant peptide. Single MHC-II allele pairs were expressed using RM3 cells