Fig. 4.
a Ex vivo induction of γH2AX and phosphorylated ATM (ATMP1981) in CD5+/CD19+ cells treated with resveratrol for 2 h. Data represent the drug-induced increase in expression of γH2AX (γH2AX IF) and activated ATM (ATMP1981 IF) compared to the respective values observed in parallel control cultures. No increase in γH2AX expression after resveratrol treatment was detected in leukemic cells of patient nos. 2, 5, 6, 7, 8, and 9. No increase in expression of ATMP1981 after resveratrol treatment was detected in leukemic cells of patient nos. 2, 3, 5, 6, 8, and 9. In patient no. 7, a slight increase in ATMP1981 expression was observed, but no increase in ATMP1981 expression was detected in patient no. 3. b Diagram showing the correlation between γH2AX and ATMP1981 expression. c Representative flow cytometric histograms showing the expression of γH2AX and ATMP1981 in CD5+/CD19+ cells treated with resveratrol for 2 h