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. 2010 Oct 30;409(2):175–188. doi: 10.1016/j.virol.2010.10.008

Fig. 7.

Fig. 7

Cell-type specific antiviral effect of ISG20 against YFV infection. 7.5-Bsr, 7.5-ISG20 and 7.5-ISG20m cells were infected with YFV Asibi strain (MOI = 0.1) (panels A and B) or YFV 17D strain (MOI = 0.1) (panel C). At indicated time points, cell-free culture supernatants were harvested and viral titers determined by fluorescent focus-forming assay (panels A and C). Data shown were representative of two independently conducted experiments. (B) Immunoblot analysis of YFV NS3, ISG20 (using anti-myc antibody), and actin expression in cell lysates under conditions in panel A. (D) HEK293-FLP-IN T-Rex cells that conditionally express ISG20 or ISG20m were cultured in the presence or absence of 1 μg/ml tetracycline for 2 days, to induce or repress ISG20/ISG20m expression, respectively, and subsequently infected with YFV 17D virus (MOI = 0.1) for 4 days. Cell-free culture supernatants were harvested and progeny viral titers were determined by fluorescent focus-forming assay (panel D). Cell lysates were harvested and subjected to immunoblot analysis of YFV NS3 (arrow head), actin (arrow) and ISG20/ISG20m (using anti-Flag antibody) proteins (panel E). Data shown are representative of two independently conducted experiments.