Fig. 9.

ISG20 does not inhibit SARS-CoV replication in Huh7.5 cells. (A) 7.5-Bsr, 7.5-ISG20 and 7.5-ISG20m cells were infected with SARS-CoV, Urbani strain (MOI = 0.01). At indicated time points, cell-free culture supernatants were harvested and viral titers determined by TCID50 assay on Vero E6 cells. Data shown were representative of two independently conducted experiments. (B) Immunoblot analysis of SARS-CoV nucleocapsid (N), ISG20/ISG20m (using anti-myc mAb), and actin expression in cell lysates under conditions of panel A.