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. 2010 Dec 28;2(1):e00313-10. doi: 10.1128/mBio.00313-10

FIG 3 .

FIG 3

The accumulation of α gene mRNAs in mock-treated cells or cells transfected with siCoREST, siREST, or control siRNAs. Replicate U251 cell cultures were mock treated or transfected with 100 pmol of siREST, siCoREST, or control siRNAs for 48 h before infection with 0.01 PFU/cell (A) or 1 PFU/cell (B) of HSV-1(F). The cells were harvested at 1, 2, 4, or 6 h (1 PFU/cell) or 1, 2, 4, 6, or 10 h (0.01 PFU/cell) after a 30-minute interval of adsorption. Total RNA was extracted, reverse transcribed, and used as a template for the quantification of the ICP27 or ICP22 transcripts accumulating in the cells. (A and B, top) The CT values of the viral mRNAs were subtracted from the respective CT values of the 18S rRNA. ΔCT values were plotted over the time points of the experiment. (A and B, bottom) First, the CT values of the viral mRNAs were subtracted from the respective CT values of the 18S rRNA to get the respective ΔCT values. Then, ΔΔCT values were obtained by subtracting ΔCT of siRNA-treated cells from ΔCT of mock-treated cells at each time point. ΔΔCT values were plotted over the time points of the experiment.