Fig. 3.

En-face preparations of Sudan-stained aortas from an apoE^−/− mouse (a) and a Watanabe heritable hyperlipidemic (WHHL) rabbit (b) injected with ¹²⁵I-MDA2. Red color (left panels in a and b) signifies the presence of neutral lipid within the atherosclerotic plaque stained with Sudan IV, and black color (right panels in a and b) in the corresponding autoradiograph signifies the presence of accumulated ¹²⁵I-MDA2 reflecting the presence of MDA-lysine epitopes. Panel C shows the relationship of ¹²⁵I-MDA2 uptake and plaque burden as measured by aortic weight. A similar relationship is present with percent atherosclerosis surface area. Panels d and e represent in vivo imaging of atherosclerotic WHHL (d) and nonatherosclerotic New Zealand white (e) rabbits with ^99mTc-MDA2. (Reprinted with permission from Tsimikas et al. [9], [10•])