TABLE 1.
Yeast strains used
| Strain number | Genotype | Reference |
|---|---|---|
| PKY3054 | MATα; lys2Δ; hmlΔ∷LEU2; cse4Δ∷kanMX + pCSE4-GFP-TRP1 | This study |
| PKY3057 | MATa; cse4Δ∷kanMX; cac1Δ∷LEU2; hir1Δ∷HIS3 + pCSE4-GFP-TRP1 | This study |
| PKY2443 | MATα; cse4Δ∷kanMX; ndc10∷NDC10-GFP∷HIS3 + pCSE4-HA-TRP1 | Sharp et al. (2002) |
| PKY2453 | MATa; cse4Δ∷kanMX; ndc10∷NDC10-GFP∷HIS3; cac1Δ∷hisG-URA3-hisG; hir1Δ∷HIS3 + pCSE4-HA-TRP1 | Sharp et al. (2002) |
| PKY3412 | MATa; ura3∷GALp-CSE4-myc12∷URA3 | This study |
| PKY3413 | MATα; cac1Δ∷LEU2; ura3∷GALp-CSE4-myc12∷URA3 | This study |
| PKY3414 | MATa; hir1Δ∷HIS3; ura3∷GALp-CSE4-myc12∷URA3 | This study |
| PKY3415 | MATa; cac1Δ∷LEU2; hir1Δ∷HIS3; ura3∷GALp-CSE4-myc12∷URA3 | This study |
| PKY4233 | MATα; ura3∷GALp-CSE4-myc12∷URA3; rpb1-1 | This study |
| PKY4323 | MATa; bar1-1; hmlΔ∷TRP1 + pHHF1-GAL10/1p-FLAG-HHT1 | This study |
| PKY4324 | MATa; bar1-1; hmlΔ∷TRP1; cac1Δ∷hisG-URA3-hisG + pHHF1-GAL10/1p-FLAG-HHT1 | This study |
| PKY4325 | MATa; bar1-1; hmlΔ∷TRP1; hir1Δ∷HIS3 + pHHF1-GAL10/1p-FLAG-HHT1 | This study |
| PKY4326 | MATa; bar1-1; hmlΔ∷TRP1; cac1Δ∷hisG-URA3-hisG; hir1Δ∷HIS3 + pHHF1-GAL10/1p-FLAG-HHT1 | This study |
| PKY2299 | MATa; cse4Δ∷kanMX + pCSE4-HA-TRP1 | This study |
| PKY2295 | MATa; cac1Δ∷hisG-URA3-hisG; cse4Δ∷kanMX; hmlΔ∷LEU2 + pCSE4-HA-TRP1 | This study |
| PKY2297 | MATa; hir1Δ∷HIS3; cse4Δ∷kanMX; hmlΔ∷LEU2 + pCSE4-HA-TRP1 | This study |
| PKY2300 | MATa; cac1Δ∷hisG-URA3-hisG; hir1Δ∷HIS3; hmlΔ∷LEU2; cse4Δ∷kanMX + pCSE4-HA-TRP1 | This study |
All strains are isogenic with the W303 (Thomas and Rothstein 1989) background and are leu2-3, 112; ura3-1; his3-11,15; trp1-1; ade2-1; can1-100 in addition to the genotypes indicated.