Figure 3.—

Socially-responsive genes CG10621, sug, and cwo are expressed in male adipose tissue. Antisense (A, C, E, G, I, K, M, and O) or sense (B, D, F, H, J, L, N, and P) RNA probes were designed to cDNA clones for CG9377 (A–D), CG10621 (E–H), sug (I–L), cwo (M and N), and egh (O and P). In situ hybridization to whole-mounted male CS tissue shows candidate gene expression in the fat body tissue (arrows) on abdominal (A, B, E, F, I, J, M–P) or head (C, D, G, H, K, and L) cuticle. Expression is indicated by light (cwo) or dark purple (CG10621, sug).