TABLE 1.
Candidate genes upregulated after 20 min of courtship
| Gene identifier | Gene name | Average fold change | GO molecular function | GO biological process |
|---|---|---|---|---|
| CG1897 | Drop (Dr) | 1.46 | Sequence-specific DNA binding | Nervous system development |
| CG3850 | sugarbabe (sug) | 1.6 | Transcription activator activity | Regulation of transcription |
| CG6494 | hairy (h) | 1.52 | Transcription repressor activity | Nervous system development |
| CG6806 | Larval serum protein 2 (Lsp2) | 1.34 | Nutrient reservoir activity | Transport |
| CG9377 | 1.7 | Serine-type endopeptidase activity | Proteolysis | |
| CG9659 | egghead (egh) | 1.36 | β-1,4-mannosyltransferase activity | Axon guidance and oogenesis |
| CG10142 | Ance-5 | 1.34 | Metallopeptidase activity | Proteolysis |
| CG10621 | 1.44 | Homocysteine S-methyltransferase activity | Unknown | |
| CG10812 | drosomycin-5 (dro5) | 1.34 | Unknown | Defense response to fungus |
| CG14489 | olf186-M | 1.26 | Unknown | Unknown |
| CG14548 | E(spl) region transcript mβHLHmβ | 1.58 | Transcription repressor activity | Nervous system development |
| CG14688 | 1.28 | Unknown | Unknown | |
| CG17100 | clockwork orange (cwo) | 1.45 | Transcription repressor activity | Regulation of circadian rhythm |
| CG17820 | female-specific independent of transformer (fit) | 1.38 | Unknown | Unknown |
| CG18477 | 1.52 | Serine-type endopeptidase activity | Proteolysis | |
| CG42370 | 1.30 | Metalloendo-peptidase activity | Proteolysis |
Comparing control male heads to courting male heads revealed that 16 genes are significantly (P < 0.001) upregulated in male heads after 20 min of courtship.