Figure 3.—

Structure of local chromosomal environments of relocated piggyBac elements. Transgenic D. melanogaster flies were created by site-specific recombination between ϕC31attB-containing plasmids (dashed line) that have the mini-white gene-containing piggyBac element being relocated (blue arrow with white box) with varying amounts of genomic DNA from the original integration site (orange, yellow, red, green, and blue rectangles) and ϕC31attP sites (light blue and gray rectangle) that were placed in the genome of D. melanogaster (thin black line) using a Mos1 gene vector with a 3xP3DsRed marker gene (thick black line with white box) (Bischof et al. 2007). The element labeled “piggyBac” was the element from line 02382 without any neighboring chromosomal DNA. The element labeled “piggyBac-NPV” was the element from the plasmid PB{RB} with 1 kb of DNA flanking each terminal inverted repeat (gray rectangle) (Thibault et al. 2004). PB{RB} was used to create most of the lines used in this study. The first 150 bp of the DNA flanking the 5′ and 3′ terminal inverted repeats of the element in plasmid PB{RB} originated from the nuclear polyhedrosis virus from which piggyBac was originally isolated (Cary et al. 1989). The features represented in this diagram were not drawn to scale.