Figure 3.—

Rev1's noncatalytic domains also function to tolerate 4-NQO lesions. (A) Cellular survival for the integrated BRCT and UBM2 rev1 mutants decreases after chronic 4-NQO exposure. Strains are rev1Δbar1Δ, YMEW1 (REV1), YMEW2 (rev1-catalytic dead), YMEW3 (rev1-BRCT), and YMEW4 (rev1-UBM2). (B) The BRCT and UBM2 rev1 mutants are also sensitive to acute 4-NQO damage. (C) The BRCT and UBM2 rev1 mutants are sensitive to UV radiation. (D) The C terminus rev1 mutant is more sensitive to chronic 4-NQO exposure than the BRCT and UBM2 rev1 mutants on plasmids. Strains for B–D are YMEW7 (rev1Δ pRS416), YMEW8 (rev1Δ pRS416-REV1), YMEW9 (rev1Δ pRS416-rev1-catalytic dead), YMEW10 (rev1Δ pRS416-rev1-BRCT), YMEW11 (rev1Δ pRS416-rev1-UBM2), and YMEW12 (rev1Δ pRS416-rev1-C-terminus). The error bars represent one standard deviation of three or more independent cultures.