TABLE 2.
Site-directed rev1 mutants used in this study
| Name in this report | Published allele name | Amino acid change | Protein location | Reference |
|---|---|---|---|---|
| Catalytic dead | rev1 Ala467–Ala468 | D467A E468A | DNA polymerase domain | Haracska et al. (2001) |
| BRCT | rev1-1 | G193R | BRCT domain | (Larimer et al. (1989); Lemontt (1971) |
| UBM2a | UBM2* | L821A P822A | UBM2 | (Guo et al. (2006b) |
| UBM2b | rev1-106 | E820A L821A P822A T823A Q824A | UBM2 | (D'Souza et al. (2008) |
| C terminus | rev1-108 | L889A V890A K891A W893A V894A | C terminus | (D'Souza et al. (2008) |
| rev1-R324K | R324K | DNA polymerase domain | This study | |
| rev1-R324T | R324T | DNA polymerase domain | This study | |
| rev1-F367L | F367L | DNA Polymerase domain | This study | |
| rev1-F441L | F441L | DNA polymerase domain | This study |
a
This allele was used for the integration into the chromosome.
b
This allele was used for pRS416.