TABLE 2.
Primers used for PCR
| Primer no. | Primer sequence | Region of hybridization | Distance from telomere VR in YSH505 (kb) |
|---|---|---|---|
| SP632 | TGTTGACATTGCGAAGAGCG | URA3 | 2.8–2.9 |
| SP633 | CACCGGGTGTCATAATCAACC | ||
| SP638 | ATCGTTATGTCCGGTGGTACC | ACT1 | |
| SP639 | TGGAAGATGGAGCCAAAGC | ||
| SP817 | GGAACGTGCTGCTACTCATCC | URA3 | 3.3–3.4 |
| SP818 | TGGTACGAACATCCAATGAAGC | ||
| SP819 | TCAGGGTAGTGCGCGTATTTG | Chr V 565042-063 | 3.7–3.8 |
| SP820 | CGTCCTTTTTCCGCCAATT | Chr V 565124-143 | |
| SP821 | GTGGGCAAATATGCGTATGATT | Chr V 564380-402 | 4.3–4.4 |
| SP822 | GGTTTCCTGTAAAAGCTGCTAAAC | Chr V 564456-480 | |
| SP823 | AAATCGTGGCAGCGGTACC | Chr V 566239-258 | 1.3–1.4 |
| SP824 | TGATATCCATGAGGTGGCGA | Chr V 566319-339 | |
| SP886 | TTGTAATGACGAGCATATCGGTG | Chr V 566603-626 | 1.0–1.1 |
| SP887 | CGTCAACAGTTCTTAATTTCGGGT | Chr V 566680-704 | |
| SP888 | CACCACATACGCGCTGATTG | Chr V 567001-021 | 0.6–0.7 |
| SP889 | GCATGTAACCAGATGAGTTATATTTGATAAT | Chr V 567072-103 | |
| SP890 | TATTTATTGCGATAGACGCACTACTTG | Chr V 567305-332 | 0.3–0.4 |
| SP891 | AAGTCTGATTGATTCTGTTGAGATTGTT | Chr V 567378-406 | |
| SP892 | GGTGGCTCTGGAGGCTCAT | Chr V 567537-556 | 0.0–0.1 |
| SP893 | CATCAACTGCACATAATTGGCG | Chr V 567617-639 | |
| SP976 | GCCCCTGGACTACGAAAACTT | MAT | |
| SP977 | ACAATTCATCATTTGCGTTCGTT | ||
| SP978 | TGGCAGAGAATCCAGATCCAA | PRP8 | |
| SP979 | ACTGCTCGCCCTAGGTTAACG | ||
| SP980 | CCCTAGGTCTTGGCAACTGG | DCC1 | |
| SP981 | ACCATCGTGGTGCTCTTTGTC |