TABLE 3.
Inducible silencing in mutant yeast backgrounds
| Strain | Relative URA3 mRNA levels (cycling induced/cycling uninduced) | G1 mRNA (blocked induced/cycling uninduced) | G2/M mRNA (blocked induced/cycling uninduced) |
|---|---|---|---|
| Wild type | 0.2 ± 0.08 | 1.0 ± 0.09 | 1.1 ± 0.13 |
| Δsir2 | 1.0 ± 0.11 | ND | ND |
| Δhtz1 | 0.2 ± 0.01 | 0.4 ± 0.00 | 0.5 ± 0.14 |
| Δsas2 | 0.1 ± 0.01 | 0.3 ± 0.14 | 0.1 ± 0.00 |
| Δset1 | 0.4 ± 0.21 | 1.1 ± 0.14 | 1.0 ± 0.28 |
| Δdot1 | 1.1 ± 0.19 | ND | ND |
| Δubp3 | 0.5 ± 0.10 | 0.9 ± 0.00 | 1.0 ± 0.00 |
| Δubp 8 | 0.3 ± 0.17 | 1.1 ± 0.00 | 1.1 ± 0.07 |
| Δubp 10 | 0.5 ± 0.14 | 1.1 ± 0.00 | 1.0 ± 0.00 |
| Δrad6 | 0.7 ± 0.07 | 1.0 ± 0.00 | 0.9 ± 0.14 |
| Δhho1 | 0.2 ± 0.08 | 1.1 ± 0.07 | 1.0 ± 0.21 |
| Δdls1 | 0.2 ± 0.07 | 1.0 ± 0.07 | 1.1 ± 0.00 |
| Δyta7 | 0.4 ± 0.07 | 1.1 ± 0.07 | 0.9 ± 0.07 |
| Δitc1 | 0.1 ± 0.00 | 1.1 ± 0.07 | 1.1 ± 0.07 |
| Δisw2 | 0.1 ± 0.00 | 1.1 ± 0.00 | 0.9 ± 0.00 |
| Δsas3 | 0.3 ± 0.07 | 1.1 ± 0.07 | 1.0 ± 0.14 |
| Δrsc2 | 0.4 ± 0.14 | 0.9 ± 0.14 | 0.9 ± 0.07 |
| Δdcc1 | 0.9 ± 0.20 | ND | ND |
| Δchl1/ctf1 | 0.5 ± 0.00 | 1.1 ± 0.00 | 1.1 ± 0.07 |
| scc1-73 (PT) | 0.5 ± 0.10 | 0.9 ± 0.05 | 1.0 ± 0.00 |
| ycs4-1 (PT) | 0.9 ± 0.19 | 1.2 ± 0.07 | 1.2 ± 0.00 |
| top2-4 (PT) | 0.9 ± 0.15 | ND | ND |
Strains bearing the indicated gene deletions were grown to steady state in raffinose media or raffinose media plus galactose and assayed for expression of the telomere-linked URA3 gene. The URA3/ACT1 ratio is shown for the galactose-induced cells relative to its uninduced control. YSH556 (lacking the galactose-inducible SIR3 gene) and YSH505 served as negative and positive controls, respectively. For G1 or G2/M experiments cells were grown in raffinose media, arrested in the cell cycle with α-factor or nocodazole, and induced with galactose (see materials and methods). Strains bearing temperature-sensitive alleles were assayed at 23° (PT) for steady-state determinations. ND, not determined.