Figure 3.—

CASK^P18 mutants display reduced levels of courtship, but not a reduction of pheromone sensitivity. (A) Loss of CASK-β lowers the courtship index (P < 0.0001 for all pairwise comparisons) and (B) increases the latency to initiate courtship (P < 0.01 for all pairwise comparisons) in both CASK^P18 and 307/313 flies. The behavioral changes in the 307/313 flies are more severe than in CASK^P18 flies in both cases. Letters signify significant differences between groups. (C) Courtship habituation assays performed either with immature male exposure (left) or with direct exposure to isolated immature male pheromone (right) produced no plasticity defect in CASK^P18 flies compared to control flies. The courtship index was so low with 307/313 flies that a valid habituation index could not be constructed for the exposure to the immature male condition, but these flies did demonstrate a significantly larger and more variable habituation index than either control or CASK^P18 flies in the direct exposure condition (P < 0.05). Statistical significance is represented by capital letters for immature male habituation and small letters for pheromone exposure habituation because ANOVA and pairwise comparisons were performed separately for these two conditions.