Figure 4.—
CASKP18 mutants display a unique locomotor deficit. (A) Locomotor traces from a 30-sec trial are shown for five groups: Con (wild-type precise excision control), Con/P18 (control/CASKP18), P18 (CASKP18), P18/313 [CASKP18/Df(3R)x313], and 307/313 [Df(3R)x307/Df(3R)x313] flies. (B) Percentage of inactivity (total percentage of time standing still) is shown for the five genotypes and demonstrates a dose-dependent increase of inactivity with loss of CASK-β. P18/313 flies have activity levels similar to those seen in CASKP18 and heterozygote flies, while the 307/313 flies appear to have a much more severe increase in inactivity, together suggesting that the overlapping deficiencies have additional elements contributing to the locomotor defect. Letters signify significant differences between groups (P < 0.05). (C) Centroid plot for MANOVA depicting the relative positions of each genotype (canonical centroids) in multivariate space. The combination of the length and direction of the vector lines indicates how strongly the behavioral parameters differentiate the genotypes.