C164-Gal4 expresses in a subset of CNS neurons. Confocal imaging was used to map C164-Gal4 expression in the brain of the adult fly with UAS-mCD8GFP. (A) A confocal stack of the anterior brain reveals low-level expression throughout much of the brain, with high expression in the antennal lobes (AL), mushroom bodies (MB), and subesophageal ganglion (SOG). Interestingly, the ellipsoid body (EB) is completely devoid of expression. There are also several other small unidentified clusters of cells that express strongly in the anterior brain (shown in schematic). (B) A confocal stack of the posterior brain appears relatively devoid of strong expression except in the pars intercerebralis (PI). Bar, 50 μm.